Journal: Bioscience Reports

Article Title: Long noncoding RNA MIAT regulates apoptosis and the apoptotic response to chemotherapeutic agents in breast cancer cell lines

doi: 10.1042/BSR20180704

Figure Lengend Snippet: The level of MIAT lncRNA was determined in 128 tumor samples and compared with 16 normal breast tissue samples. ( A ) MIAT expression showed significant increase in the full data sets of breast tumors compared with normal. ( B ) Corresponding scattergrams of these data are shown. ( C ) Analysis of the data revealed that there is a highly significant up-regulation of MIAT lncRNA in stage I–II disease. ( D ) Stratification of MIAT expression levels according to the molecular subtypes showed variation in MIAT expression. MIAT expression was not changed in the triple positive (ER, PR, and HER2 positive) samples, significantly increased in ER, PR +ve, HER –ve, and TNBC breast cancer subtypes (Mann–Whitney Rank Sum Test (A and B) and Kruskal–Wallis one-way ANOVA on ranks (C and D); ** P <0.01 and **** P < 0.0001; n =16 (normal), 128 (breast adenocarcinoma specimens)).

Article Snippet: To further investigate whether the mRNA expression levels of MIAT exhibit changes in breast cancer, we utilized breast cancer cDNA arrays (BCRT101, BCRT102, and BCRT104) from OriGene Inc and determined MIAT expression levels in breast cancer and normal tissues.

Techniques: Expressing, MANN-WHITNEY

Journal: Oncotarget

Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

doi: 10.18632/oncotarget.10063

Figure Lengend Snippet: qPCR using a3-specific primers A. or a4-specific primers B. was performed on the Origene breast cancer cDNA array II as described under Materials and Methods. Data was analyzed using the ΔΔC t method. Values represent a3 or a4 expression levels relative to the first normal breast tissue sample (sample 1, indicated by the red dotted line) and are the mean of two separate experiments. Error bars indicate S.E. In the a3 graph, *, p < 0.05 relative to the normal samples and **, p < 0.11 relative to the normal samples.

Article Snippet: To measure a3 mRNA expression in human breast cancer samples, qRT-PCR was performed using a3-specific primers on a breast cancer cDNA panel (Origene).

Techniques: Expressing